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vx 702 mce cat  (MedChemExpress)


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    MedChemExpress vx 702 mce cat
    Vx 702 Mce Cat, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vx 702 mce cat/product/MedChemExpress
    Average 93 stars, based on 5 article reviews
    vx 702 mce cat - by Bioz Stars, 2026-03
    93/100 stars

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    ( A, B ) Western blot and densitometric analysis of the phosphorylated proteins p-p38, p-ERK and p-JNK following SSc lung ( A ) and skin ( B ) MF treatment with forskolin (20 µM) for 6 h. Phosphorylated proteins were normalized to total level of their respective proteins. ( C ) Western blot and densitometric analysis of the fibrosis-associated genes Col1A1 and αSMA following SSc lung and skin MF treatment with SB203580 (20 µM) for 96 h. ( D ) αSMA stress fibers were identified by immunofluorescence microscopy using an anti-αSMA-FITC-conjugated antibody (using the same protocol in C ). Nuclei were stained with DAPI. Data points represent distinct patient-derived cell lines. Significance for densitometric data ( n = 5-7) in A and B was determined and by 2-tailed paired t-test and by one-way ANOVA in C . * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.
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    ( A, B ) Western blot and densitometric analysis of the phosphorylated proteins p-p38, p-ERK and p-JNK following SSc lung ( A ) and skin ( B ) MF treatment with forskolin (20 µM) for 6 h. Phosphorylated proteins were normalized to total level of their respective proteins. ( C ) Western blot and densitometric analysis of the fibrosis-associated genes Col1A1 and αSMA following SSc lung and skin MF treatment with SB203580 (20 µM) for 96 h. ( D ) αSMA stress fibers were identified by immunofluorescence microscopy using an anti-αSMA-FITC-conjugated antibody (using the same protocol in C ). Nuclei were stained with DAPI. Data points represent distinct patient-derived cell lines. Significance for densitometric data ( n = 5-7) in A and B was determined and by 2-tailed paired t-test and by one-way ANOVA in C . * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.
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    ( A, B ) Western blot and densitometric analysis of the phosphorylated proteins p-p38, p-ERK and p-JNK following SSc lung ( A ) and skin ( B ) MF treatment with forskolin (20 µM) for 6 h. Phosphorylated proteins were normalized to total level of their respective proteins. ( C ) Western blot and densitometric analysis of the fibrosis-associated genes Col1A1 and αSMA following SSc lung and skin MF treatment with SB203580 (20 µM) for 96 h. ( D ) αSMA stress fibers were identified by immunofluorescence microscopy using an anti-αSMA-FITC-conjugated antibody (using the same protocol in C ). Nuclei were stained with DAPI. Data points represent distinct patient-derived cell lines. Significance for densitometric data ( n = 5-7) in A and B was determined and by 2-tailed paired t-test and by one-way ANOVA in C . * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.
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    Image Search Results


    ( A, B ) Western blot and densitometric analysis of the phosphorylated proteins p-p38, p-ERK and p-JNK following SSc lung ( A ) and skin ( B ) MF treatment with forskolin (20 µM) for 6 h. Phosphorylated proteins were normalized to total level of their respective proteins. ( C ) Western blot and densitometric analysis of the fibrosis-associated genes Col1A1 and αSMA following SSc lung and skin MF treatment with SB203580 (20 µM) for 96 h. ( D ) αSMA stress fibers were identified by immunofluorescence microscopy using an anti-αSMA-FITC-conjugated antibody (using the same protocol in C ). Nuclei were stained with DAPI. Data points represent distinct patient-derived cell lines. Significance for densitometric data ( n = 5-7) in A and B was determined and by 2-tailed paired t-test and by one-way ANOVA in C . * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.

    Journal: bioRxiv

    Article Title: Distinct cAMP regulation in scleroderma lung and skin myofibroblasts governs their dedifferentiation via p38α inhibition

    doi: 10.1101/2025.02.26.640163

    Figure Lengend Snippet: ( A, B ) Western blot and densitometric analysis of the phosphorylated proteins p-p38, p-ERK and p-JNK following SSc lung ( A ) and skin ( B ) MF treatment with forskolin (20 µM) for 6 h. Phosphorylated proteins were normalized to total level of their respective proteins. ( C ) Western blot and densitometric analysis of the fibrosis-associated genes Col1A1 and αSMA following SSc lung and skin MF treatment with SB203580 (20 µM) for 96 h. ( D ) αSMA stress fibers were identified by immunofluorescence microscopy using an anti-αSMA-FITC-conjugated antibody (using the same protocol in C ). Nuclei were stained with DAPI. Data points represent distinct patient-derived cell lines. Significance for densitometric data ( n = 5-7) in A and B was determined and by 2-tailed paired t-test and by one-way ANOVA in C . * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.

    Article Snippet: The pan-p38 inhibitor SB203580 (20 μM) and p38α inhibitor VX-702 (50 μM) were purchased from Cayman Chemicals (13067) and Selleckchem (HY-10401), respectively.

    Techniques: Western Blot, Immunofluorescence, Microscopy, Staining, Derivative Assay

    ( A ) qPCR data representing the relative expression of the genes encoding p38α, p38β, p38γ and p38δ in SSc lung and skin MFs. ( B ) Western blot and densitometric analysis of the fibrosis-associated genes Col1A1 and αSMA following SSc lung and skin MF treatment with the isoform-specific p38α inhibitor VX-702 (50µM) for 96 h. ( C ) αSMA stress fibers were identified by immunofluorescence microscopy using an anti-αSMA-FITC-conjugated antibody (using the same protocol in B ). Nuclei were stained with DAPI. Data points represent distinct patient-derived cell lines. Significance for data in A ( n = 8) was determined by 2-tailed unpaired or paired t-test where appropriate and by one-way ANOVA in B (n = 6). * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.

    Journal: bioRxiv

    Article Title: Distinct cAMP regulation in scleroderma lung and skin myofibroblasts governs their dedifferentiation via p38α inhibition

    doi: 10.1101/2025.02.26.640163

    Figure Lengend Snippet: ( A ) qPCR data representing the relative expression of the genes encoding p38α, p38β, p38γ and p38δ in SSc lung and skin MFs. ( B ) Western blot and densitometric analysis of the fibrosis-associated genes Col1A1 and αSMA following SSc lung and skin MF treatment with the isoform-specific p38α inhibitor VX-702 (50µM) for 96 h. ( C ) αSMA stress fibers were identified by immunofluorescence microscopy using an anti-αSMA-FITC-conjugated antibody (using the same protocol in B ). Nuclei were stained with DAPI. Data points represent distinct patient-derived cell lines. Significance for data in A ( n = 8) was determined by 2-tailed unpaired or paired t-test where appropriate and by one-way ANOVA in B (n = 6). * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.

    Article Snippet: The pan-p38 inhibitor SB203580 (20 μM) and p38α inhibitor VX-702 (50 μM) were purchased from Cayman Chemicals (13067) and Selleckchem (HY-10401), respectively.

    Techniques: Expressing, Western Blot, Immunofluorescence, Microscopy, Staining, Derivative Assay